IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS
By: Nwakaego, Nwokolo Lorreta
.
Contributor(s): Chibuike, Onyekwelu Kenechukwu.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(1).Description: 67-71p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
This study investigated the
in vitro
antioxidant and free radical potentials of methanol extracts of
Uvaria chamae
leaves and roots
.
Method
s:
Fresh
Uvaria chamae
leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids
,
antioxidant
and tannin contents
, DPPH, hydroxyl, and superoxide rad
ical scavenging properties
of the extracts were determined using standard methods.
Result
s:
In vitro
antioxid
ant potentials revealed that
methanol extract of
Uvaria chamae
leaves
contains vitamin A
(4871±79.21 I
. U
) and vitamin C
(1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I
. U
) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin
E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1
-diphenyl
-2-picrylhydrazyl
radical (DPPH) in a concentration dependent manner with the
correlation coefficient (R
2
) of 0.839 and effective concentration (EC
50
) of
31.19 μg/ml
, while the root extract scavenged DPPH with R
2
of0.778 and
EC
50
of
14.00 μg
/ml
. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC
50
of
5.93 μg/ml
and 719.45 μg/ml;
107.89 μg/ml
and 912.01 μg
/ml
respectively compared to the
EC
50
of ascorbic standard (
30.27 μg/ml
) and
EC
50
Conclusio
n:
The
leaves and roots of
Uvaria
chamae
are
rich in natural antioxidants that can be exploited in the treatment of diseases related to
oxidative stress.
of vitamin E standard
(106.66μg
/ml
) respectively. The
leaf extract showed significantly higher (
p
<0.05) anti radical power (ARP) of superoxide (0.17) comp
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020938 |
Objective:
This study investigated the
in vitro
antioxidant and free radical potentials of methanol extracts of
Uvaria chamae
leaves and roots
.
Method
s:
Fresh
Uvaria chamae
leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids
,
antioxidant
and tannin contents
, DPPH, hydroxyl, and superoxide rad
ical scavenging properties
of the extracts were determined using standard methods.
Result
s:
In vitro
antioxid
ant potentials revealed that
methanol extract of
Uvaria chamae
leaves
contains vitamin A
(4871±79.21 I
. U
) and vitamin C
(1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I
. U
) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin
E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1
-diphenyl
-2-picrylhydrazyl
radical (DPPH) in a concentration dependent manner with the
correlation coefficient (R
2
) of 0.839 and effective concentration (EC
50
) of
31.19 μg/ml
, while the root extract scavenged DPPH with R
2
of0.778 and
EC
50
of
14.00 μg
/ml
. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC
50
of
5.93 μg/ml
and 719.45 μg/ml;
107.89 μg/ml
and 912.01 μg
/ml
respectively compared to the
EC
50
of ascorbic standard (
30.27 μg/ml
) and
EC
50
Conclusio
n:
The
leaves and roots of
Uvaria
chamae
are
rich in natural antioxidants that can be exploited in the treatment of diseases related to
oxidative stress.
of vitamin E standard
(106.66μg
/ml
) respectively. The
leaf extract showed significantly higher (
p
<0.05) anti radical power (ARP) of superoxide (0.17) comp
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